Padmashree College of
Medical Laboratory Technology

India

Syllabus for 1st year B.Sc. in Medical Laboratory Technology

PATHOLOGY – 1

 

PRINCIPLES OF PATHOLOGY AND MICROBIOLOGY

I- THEORY

A- Introduction to Histopathology:

1. Receiving of specimens

2. Grossing Techniques

3. Various fixatives - Mode of action, Indications, Preparations

4. Decalcification of calcified tissue before sectioning

5. Processing of tissues for routine paraffin sections and other methods of embedding

6. Staining principles, preparation of reagents and techniques

7. The use of Microscope, screening techniques

B- Laboratory Organization:

1. Reception of specimen, dispatch of reports, 'Records keeping' coding the lesions of cases.

2. Follow up program, quality control of technique etc.,

C- Clinical Pathology:

1. Urine examination

a. Physical

b. Chemical

c. Microscopic

2. Examination of body fluids, cell counts

3. Semen analysis

4. Cerebrospinal Fluid (CSF)

5. Stool examination

 

II- LABORATORY EXERCISES

A. Examination of Urine samples:

1. Physical examination: volume, color, odor, appearance, specific gravity and PH.

2. Chemical examination:

a. Protein: - Heat and acetic acid test

b. Sulfosalicylic acid method

c. Reducing Sugar – Benedict test

d. Ketone bodies - Rothera test

e. Bile pigment - Fouchet method

f. Bile salt - Hays test

g. Blood - Benzidine test

h. Urobilinogen & Porphobilinogen - Ehrlich aldehyde and schwartz test

i. Bence Jones Protein

B. Examination of Stool samples:

1. Physical examination

2. Chemical examination

a. Occult blood

b. Reducing sugar

3. Microscopic examination for ova, cysts, crystals and fat globules

C. Examination of Semen Samples

1. Physical examination

2. Chemical examination

3. Microscopic examination

D. Examination of Cerebrospinal Fluid (CSF)

1. Physical examination

2. Chemical examination

3. Microscopic examination

E. Examination of Body Fluids (pleural, pericardial and peritoneal)

1. Physical examination

2. Chemical examination

3. Microscopic examination

F. Examination of Synovial Fluid

1. Physical examination

2. Chemical examination

3. Microscopic examination

 

HISTOPATHOLOGY (Part 1)

A. Grossing techniques

B. Preparation of various fixatives.

C. Decalcification of calcified tissue before sectioning

D. Processing of tissue for routine paraffin sections and other methods of embedding.

 

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